grgds  (Biosynth Carbosynth)

Journal: ACS Omega

Article Title: In Situ Monitoring of Linear RGD-Peptide Bioconjugation with Nanoscale Polymer Brushes

doi: 10.1021/acsomega.6b00450

Figure Lengend Snippet: In situ ATR-FTIR spectra of GRGDS bioconjugation (0.1 mg/mL) onto polymer brushes. (a) GRGDS covalent binding to PAA brush; (b) GRGDS covalent binding to PNIPAAm–PAA brush with a ratio of 80:20. The experiment was performed in five discrete steps. Step I: swelling of the brush in MES; step II: EDC/NHS activation; step III: GRGDS conjugation; step IV: washing with PBS; and step V: quenching with acetate buffer at pH 4.0. The spectra were dynamically recorded over the entire time frame of each experimental step with a time step of t = 5 min. Curves were shifted along the vertical axis for better display.

Article Snippet: Fibronectin active fragment Gly-Arg-Gly-Asp-Ser (GRGDS) was obtained from Peptides International Inc. (Louisville, KY and Canada).

Techniques: In Situ, Polymer, Binding Assay, Activation Assay, Conjugation Assay

Journal: ACS Omega

Article Title: In Situ Monitoring of Linear RGD-Peptide Bioconjugation with Nanoscale Polymer Brushes

doi: 10.1021/acsomega.6b00450

Figure Lengend Snippet: Quantification of the GRGDS peptide bound to a homo-PAA brush and to binary PNIPAAm–PAA brushes with ratios 20:80 and 80:20 by acidic hydrolysis and subsequent HPLC analysis. Comparison of physisorption and chemisorption following the optimized Protocol VII. Initial GRGDS concentration was 0.1 mg/mL. The physisorbed amounts were below the detection limit (n.d.).

Article Snippet: Fibronectin active fragment Gly-Arg-Gly-Asp-Ser (GRGDS) was obtained from Peptides International Inc. (Louisville, KY and Canada).

Techniques: Comparison, Concentration Assay

Journal: ACS Omega

Article Title: In Situ Monitoring of Linear RGD-Peptide Bioconjugation with Nanoscale Polymer Brushes

doi: 10.1021/acsomega.6b00450

Figure Lengend Snippet: ATR-FTIR analysis of GRGDS bioconjugation (0.1 mg/mL) onto homo-PAA brush surfaces. Comparison of exemplarily selected EDC/NHS chemisorption protocols according to Table . (a) Final surface state after GRGDS chemisorption. Curves were shifted along the vertical axis for better display. Rinsing with acetate solution of pH 4.0 was performed before the analysis. (b) Absorbance units refer to normalized relative band intensities of the amide I and amide II as well as the sum of the amide I and amide II vibration frequency bands.

Article Snippet: Fibronectin active fragment Gly-Arg-Gly-Asp-Ser (GRGDS) was obtained from Peptides International Inc. (Louisville, KY and Canada).

Techniques: Comparison

Journal: ACS Omega

Article Title: In Situ Monitoring of Linear RGD-Peptide Bioconjugation with Nanoscale Polymer Brushes

doi: 10.1021/acsomega.6b00450

Figure Lengend Snippet: In situ VIS–SE monitoring of GRGDS bioconjugation (0.1 mg/mL) onto homo-PAA brushes. (a) Detection of PAA layer thickness and refractive index changes during bioconjugation. (b) The time-dependent GRGDS amount (mg/m 2 ) chemisorbed onto the PAA brush surfaces comparing Protocols II and VI. In situ ellipsometry measurements are performed in a batch cell without stirring.

Article Snippet: Fibronectin active fragment Gly-Arg-Gly-Asp-Ser (GRGDS) was obtained from Peptides International Inc. (Louisville, KY and Canada).

Techniques: In Situ, Refractive Index

Journal: ACS Omega

Article Title: In Situ Monitoring of Linear RGD-Peptide Bioconjugation with Nanoscale Polymer Brushes

doi: 10.1021/acsomega.6b00450

Figure Lengend Snippet: Quantitative VIS–SE analysis of the amount of GRGDS, Γ (mg/m 2 ), bound to homo-PAA brushes as a function of GRGDS concentration. Comparison of the GRGDS bioconjugation efficiency between the less optimal Protocol II and the most optimal Protocol VII as well as with the GRGDS physisorption results under the same conditions as for conjugation.

Article Snippet: Fibronectin active fragment Gly-Arg-Gly-Asp-Ser (GRGDS) was obtained from Peptides International Inc. (Louisville, KY and Canada).

Techniques: Concentration Assay, Comparison, Conjugation Assay

Journal: ACS Omega

Article Title: In Situ Monitoring of Linear RGD-Peptide Bioconjugation with Nanoscale Polymer Brushes

doi: 10.1021/acsomega.6b00450

Figure Lengend Snippet: EDC/NHS Chemisorption of GRGDS Peptide onto Homo-PAA Brush Surfaces a , b

Article Snippet: Fibronectin active fragment Gly-Arg-Gly-Asp-Ser (GRGDS) was obtained from Peptides International Inc. (Louisville, KY and Canada).

Techniques:

Journal: ACS Omega

Article Title: In Situ Monitoring of Linear RGD-Peptide Bioconjugation with Nanoscale Polymer Brushes

doi: 10.1021/acsomega.6b00450

Figure Lengend Snippet: Summary of All Comparatively Investigated Reaction Parameters Tested for the GRGDS Bioconjugation via EDC/NHS Chemisorption

Article Snippet: Fibronectin active fragment Gly-Arg-Gly-Asp-Ser (GRGDS) was obtained from Peptides International Inc. (Louisville, KY and Canada).

Techniques: Activation Assay

Journal: Acta biomaterialia

Article Title: “A two-component pre-seeded dermal-epidermal scaffold”

doi: 10.1016/j.actbio.2014.08.029

Figure Lengend Snippet: Peptide conjugation to HA-CHO. A) Chemical structure of HA-CHO, the fibronectin-derived peptide sequence, GRGDS, and the peptide-modified, HA-CHO-GRGDS; (* denotes methylene protons in the side chains of arginine and aspartate in the GRGDS peptide, corresponding to asterisked peak at ~2.6-2.8 ppm in panel B, § denotes the methyl protons of HA-CHO at 1.8 ppm). B) 1H-NMR spectra showed that approximately 10% of the available carboxylic acids in HA were modified with the peptide (* indicates the conjugated peptide). C) Gel permeation chromatography showed an increase in molecular weight of the HA-CHOGRGDS conjugate relative to the molecular weight of HA-CHO.

Article Snippet: Fibronectin active fragment, Gly-Arg-Gly-Asp-Ser, was purchased from Peptides International (Louisville, KY, USA).

Techniques: Conjugation Assay, Derivative Assay, Sequencing, Modification, GPC Assay, Molecular Weight